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The Lambert Instruments Fluorescence lifetime Attachment (LIFA) is a fast frequency-domain fluorescence lifetime imaging microscopy system compatible with Leica, Nikon, Olympus and Zeiss widefield fluorescence microscopes.
You can see the LIFA at one of our Test Centers.
The LIFA - Lambert Instruments Fluorescence lifetime Attachment - is a camera-based fast fluorescence lifetime imaging microscopy system that operates in the frequency domain and is compatible with Leica, Nikon, Olympus, TILL and Zeiss fluorescence microscopes.
The well-established homodyne detection technology together with the massive parallelism of the state-of-the-art intensified camera allows near instantaneous acquisition of full field lifetime images. The widefield system includes a Multi-LED modulated light source. Its high-power LEDs can be modulated in a broad frequency range, resulting in good lifetime sensitivity and high accuracy. The components of the LIFA are the TRiCAM modulated intensified CCD camera, a dual signal generator, and the LI-FLIM software package.
The LIFA is easy to install - within the hour - and very easy to operate. The LIFA is in addition well suited for high-content screening applications. The LIFA system has been judged "easy and highly quantitative" for a.o. FLIM-FRET studies.
Fast fluorescence lifetime imaging microscopy, up to two lifetime images per second
Non-phototoxic illumination offered by the Multi-LED (NEW)
Lifetime sensitivity 0-300 ns
Lifetime accuracy 30 ps r.m.s.
High quantum efficiency with the Gen III GaAs image intensifier (Optional)
FRET efficiency mapping
Time-lapses
Multi-frequency acquisition and analysis for separation of multiple lifetimes
Polar (Phasor) plot inspection and separation of multiple lifetimes
Easy integration into specialized image analysis pipelines through ActiveX
Export to Metamorph, ImageJ, Matlab
Images can be exported as BMP, TIFF
Statistics and histograms can be exported to MS Excel
Lambert Instruments provides the following LIFA models, which cover a wide range in spectral sensitivity and in lifetime sensitivity. Each model is based on a specific high-resolution Gen II or Gen III image intensifier, see also our fluorescence lifetime imaging microscopy properties of modulated intensifiers document.
Model | Photocathode | Modulation frequency | Lifetime sensitivity |
| LIFA Gen II S20 | S20 | 1-120 MHz | 0-300 ns |
| LIFA Gen II S25 | S25 | 1-120 MHz | 0-300 ns |
| LIFA Gen III GaAs | GaAs | 1-120 MHz | 0-300 ns |
| LIFA X Gen II S20 | S20 | 0-100 kHz, 1-120 MHz | 0 ns - 1 ms |
| LIFA X Gen II S25 | S25 | 0-100 kHz, 1-120 MHz | 0 ns - 1 ms |
| LIFA X Gen III GaAs | GaAs | 0-100 kHz, 1-120 MHz | 0 ns - 1 ms |
| LIFA P Gen II S20 | S20 | 0-100 kHz | 100 ns - 1 ms |
| LIFA P Gen II S25 | S25 | 0-100 kHz | 100 ns - 1 ms |
| LIFA P Gen III GaAs | GaAs | 0-100 kHz | 100 ns - 1 ms |
| LIFA P Gen III GaAsP | GaAsP | 0-100 kHz | 100 ns - 1 ms |
Spectral sensivity as a function of wavelength for each of the photocathodes is shown below.
For more information, please refer to our technologies page on the operating principle of image intensifiers for lifetime imaging.
Options
Nikon Ti Eclipse.
Prior Scientific ProScan III (Motorized stage system for automated FLIM acquisition at a series of user-defined XYZ positions.)
Mad City Labs Nano-F100 (Nanopositioning system, a z-stepper.)
The LIFA has the following applications:
Molecular interactions
Protein conformation
Biosensors
Oxygen concentration imaging in cells and tissue
NADH/FAD fluorescence dynamics
Viscosity imaging
Membrane dynamics
Membrane trafficking
LED inspection
Crude oil characterisation
